determined force, instead of amplitude parameters usually

attributed to this type of assay [20], since reproducibility can

be compromised due slight changes in sample dimensions. By

this way, submitting each specimen to a specific force of 1 N

will

guarantee

an

accurate

homogeneous

comparison

between them.

12. The 1 N was applied 3 times per day, each time during 1 h, with

each new application preceded by a 1 h constraint-free rest.

Acknowledgments

The authors thanks to UFSCar-Sorocaba/PPGBMA for support

and to CAPES grant 1724839.

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